Biochem/physiol Actions

Reversible: no

Kd = 126 nM against GSK-3β

Product does not compete with ATP.

Cell permeable: yes

Primary TargetGSK-3β

General description

A cell-permeable pyrrolopyrimidine compound that acts as a potent inhibitor of GSK-3β (IC₅₀ = 30 nM). Binds to GSK-3β with high-affinity (K_d = 126 nM) and increases the level of β-catenin, a downstream substrate of GSK-3β in the Wnt signaling pathway. Shown to selectively induce neuronal differentiation in both pluripotent murine embryonal carcinoma cells (P19; 30-40% at 1 µM) and embryonic stem cells (D3; 50-60% at 400 nM).

A cell-permeable and potent inhibitor of GSK-3β (IC₅₀ = 30 nM). Binds to GSK-3β with high-affinity (K_d = 126 nM), leading to an increase in the level of β-catenin, a downstream substrate of GSK-3β in the Wnt signaling pathway. Shown to selectively induce neuronal differentiation in both pluripotent murine embryonal carcinoma cells (P19; 30-40% at 1 µM) and embryonic stem cells (D3; 50-60% at 400 nM).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Ding, S., et al. 2003. Proc. Natl. Acad. Sci. USA100, 7632.

Packaging

1, 5 mg in Plastic ampoule

Packaged under inert gas

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Warning

Toxicity: Carcinogenic / Teratogenic (D)